Microbiology
| Code |
Sample |
Qty |
Container |
| 1 |
Blood |
2ml |
Vacutainer tube (plain) |
| 2 |
Blood |
5-10ml |
Routine Blood culture
bottle (Adult / Paediatric) |
| 3 |
Stool
(Fresh Sample) |
Adequate
Qty. |
Clean and leak proof,
screw capped container |
| 4 |
Sputum |
3-5 ml |
Sterile Container |
| 5 |
Bronchial aspirate
E.T. Aspirates |
5-10 ml |
Suction Extractor or
Sterile Container |
| 6 |
CSF |
2-3 ml |
Specified small screw
capped sterile vial |
| 7 |
Tissue, FNAC,
Pus |
Adequate
Qty. |
Sterile container |
| 8 |
Urine (for AFB) |
min. 100 ml. |
Sterile container
(Whole of MorningSample) |
| 9 |
Nail, Skin, Hair |
More than
3 pcs. |
wrapped in clean paper |
| 10 |
Urine |
20-30 ml. |
Sterile container |
| 11 |
Body fluids |
5-10 ml. |
Sterile container |
| 12 |
Swabs |
2 Swabs |
Sterile tube with swabs |
| 13 |
Urine |
10 ml |
Clean Container
(Morning mid streamSample Preferred) |
| BacT/Alert cultures |
|
|
| 14 |
Blood /Body fluids |
5-10 ml. |
BacT/Alert FAN
Aerobic / Anaerobic Bottles |
|
|
3-5 ml.
Children |
Pedi BacT Bottles |
| Anaerobic Culture |
|
|
| 15 |
Pus, Urine |
Adequate
Qty. |
in a sterile syringe,
excluding air bubbles &plugged with a rubberbung |
|
| 1) |
URGENT
Reports** |
Sample
Code |
| |
Hanging
Drop preparation |
3 |
| |
Trophozoites
of E. histolytica in liver aspirates* |
7 |
| |
Albert's
stain for C. diphtheriae |
12 |
| |
Gram
Stain for Clostridia |
15 |
| |
Pregnancy
report in operative cases |
13 |
| |
*
Sample must reach the lab within half an hour.
** Provisional Report given by technician on duty, with hr. |
|
| 2) |
BacT/Alert Automated cultures of Blood, Body Fluids
(excluding Urine) Earliest
Report generated after 12 hours. However, status of the sample
can be ascertained 6 hourly from the Lab. |
14 |
| 3)
|
Reports by 5 PM on the same day
(on samples received upto 2
PM on working days) |
|
| |
Gram
Stain |
4,
5, 6, 7, 11, 12 |
| |
Kinyoun's
Stain |
4,
5, 7, 3 |
| |
Giemsa
Stain |
5 |
| |
Fungal
Stain (KOH preparation)* |
4,
5, 6, 7, 10, 11 |
| |
India
Ink Preparation |
6 |
| |
Crypto-LA |
6 |
| |
Pregnancy
Test |
13 |
| |
RA,
CRP, ASO |
1 |
| |
HBsAg |
1 |
| |
HIV
ELISA |
1 |
| |
Hepatitis
C Virus (HCV) ELISA |
1 |
| |
Dengue
ELISA |
|
| |
*
24 h in case of thick specimen material where extended period
for d---- is required. |
1 |
| 4) |
Reports by 5 PM next working day |
|
| |
All
samples in 3) received after 2 PM |
|
| |
AFB:
ZN Stain, Direct Fluorescence |
4,
5, 6, 7, 8, 11, 12 |
| |
Urine
Culture - Negative reports |
10 |
| |
Sputum,
Throat Swab Negative Cultures |
4,
12 |
| |
E-test
MIC on isolates |
|
| |
HBsAg
Confirmatory test |
1 |
| |
HIV
Confirmatory test |
1 |
| |
VDRL,
WIDAL |
1 |
| 5) |
Reports after 48 - 72 hours. |
|
| |
Routine
aerobic culture of Blood,
Pus, Body fluids, HVS, Stool
BAL, ET and respiratory aspirates.
Positives: 72 hours / Negatives: 48 hours; |
5 |
| |
Positive
Cultures of Urine, Sputum, Throats swab with Antibiotic
sensitivity. |
10,
4, 12 |
| |
Mantoux
test (Patient has to come for the
evaluation) |
|
| 6) |
Reports after 4 weeks: Fungal Culture |
4,
5, 6, 7, 9 |
| 7) |
AFB Cultures: Negative: 6-8 weeks |
10,11,12 |
| |
Positive
with sensitivity: 12-16 weeks |
|
| 8) |
AFB
culture (Rapid method) |
|
|
| Test |
Sample
Receipt Deadline |
Report
at 5 p.m. |
| HBsAg |
10.30
AM |
Daily
Negative same day Positive with Confirmatory test next day |
| Anti
HBsAg |
Friday
8 PM |
Sat. |
| IgM
HBc |
Wed.
8 PM |
Thurs. |
| HBeAg |
Wed.
8 PM |
Thurs. |
| Antibody
HbeAg |
Wed.
8 PM |
Thurs. |
| HCV
Antibody |
10.30
AM Daily |
Daily |
| HIV
Antibody |
2
PM Daily |
Negative
Same day Confirmatory Test on Positives, next day |
| IgM
HAV |
Thurs.
8 PM |
Fri. |
| IgM
HEV |
Thurs.
8 PM |
Friday |
TORCH
IgG (Toxoplasma
Rubella,
CMV, Herpes) |
Tues.
8 PM |
Wed. |
TORCH
IgM (Toxoplasma,
Rubella,
CMV, Herpes) |
Mon.
8 PM |
Tues. |
| Cysticercosis
Antibody |
Sun.
8 PM |
Mon. |
Echinococcus
Antibody (Hydatid
Serology) |
Sun.
8 PM |
Mon. |
| E.
histolytica IgG (Amoebic Serology) |
Fri.
8 PM |
Sat. |
| Dengue |
Daily |
- |
| Chlamydia
Antigen |
Approx.
2 weeks |
|
| I.F.
for P. carinii |
Approx.
2 weeks |
|
| C3
C4 |
Fri.
8 PM |
Mon. |
| Anti-TB
IgM |
Sun.
8 PM
Thurs. 8 PM |
|
|
- Routine reporting time may alter under special circumstances
on scientific basis.
- Special Immunology investigations on a holiday
may bepreponed or post-poned by a day depending on the workload.
- Mantoux Test: The patient has to come to the
laboratory after 48-72 hours (as per appointment) for recording the observations
& generation of report.
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Sir Ganga Ram Hospital, Rajinder Nagar, New Delhi 110060, INDIA
Tel: 2575000 Fax: 25861002 Email: gangaram@sgrh.com
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