Faculty
Dr. M. Bhargava
(Chairperson)
Dr.
Shashi Narayan
Dr.
Dharma R Choudhary
(Clinical Haematology)
Dr.
Amrita Saraf
Dr.
Anil Handoo
Dr.
Pratibha Kumari |
 |
Introduction
From a relatively
small Haematology laboratory to a composite Department of Haematology, with
clinical and laboratory faculty working together under one roof for better
service, resident training and research activities, we indeed have come a long
way. Our mission is to provide state of the art investigative facilities for the
comprehensive diagnosis of haematologic diseases and the highest quality of
clinical care for our patients with the latest available therapeutic tools. In
addition, to impart training and mentor the next generation of academic
haematologists,through our Diplomate of National Board (DNB) programme, is a
high priority. For Ph.D, Guru Gobind Singh Indraprastha University has accorded
the status of Approved Research Centre to Sir Ganga Ram Hospital and the head of
the dept. is an approved PhD guide. Department has its own SOPs and protocols
and has accreditation for the laboratory services from the National
Accreditation Board for Testing Laboratories (NABL). The NABH accreditation is
underway.
The department
participates in and holds many Continuing Medical Education (CME) programmes and
conferences.
Various research activities are currently going on, which include Thalassemia &
haemoglobinopathies, haemopoietic malignancies and stem cells. The department
has five faculty members, one scientist, two senior residents (DNB students) &
24 highly competent technologists to man the laboratories.
DNB Programme
Information
Eligibility:
-
refer to National
Board of Examinations website: http://www.natboard.edu.in/
-
refer to Medical
Council of India website: www.mciindia.org/
No of seats: 02
Admission procedure:
refer to National Board of Examinations website: http://www.natboard.edu.in/
Services provided
Working hours
Routine : 8.00 A.M. to 5.00 P.M.
Emergency : Round-the-clock
Routine
investigations
Haematology
(Beckman Coulter LH 755 & LH 750
analysers, StaRRsed ESR instrument, ACL9000, ACL Advance Coagulometers)
|
Complete blood counts |
Hb, Hct, TRBC, MCV,
MCH, MCHC, RDW, Platelet count , TLC, MPV and automated 5 - part
differential & reticulocyte counts (Beckman Coulter LH 755 & LH 750
analysers)
Absolute
eosinophil, count (AEC) , Erythrocyte sedimentation rate (ESR), peripheral
blood film examination for morphology and parasites |
|
Bone marrow examination |
More than 1000 bone marrow specimens
(aspirates, imprints and biopsies) are received annually. Several rare
disorders are diagnosed each year. Immunohistochemistry is routinely
performed on biopsy specimens |
|
Haemostatic disorders |
Instrumentation Laboratory ACL
Advance, ACL 9000 & ACL 200 fully automated coagulometers |
|
Haemorrhagic disorders |
Prothrombin time (PT)*, Activated
partial thromboplastin time (APTT), Thrombin time (TT) assays of factor VIII
and IX, Platelet count, Fibrinogen estimation, Quantitative D-dimer
(Fibrinogen degradation products), Bleeding Time (BT), Clot retraction test,
Clot solubility test (factor-XIII), APTT mixing experiment, PT mixing
experiment. (*=PT is reported in INR), Factor Xa assays |
|
Thrombophilia |
For antiphospholipid syndrome and
lupus anticoagulants: APTT with lupus sensitive reagent, APTT - dependent
inhibitor screen, Dilute Russell Viper Venom Time (dRVVT), Kaolin clotting
time (KCT), Anti-cardiolipin antibodies (ANA), Anti-ds-DNA antibodies ProC
global screening test; Protein C, Protein S & Antithrombin (clot based
functional assays), Factro V leiden (APC-R) estimation |
|
Haemolytic anemias |
Screening/testing for beta -thalassemia
& hemoglobinopathies by HPLC (BioRad Variant I) and haemoglobin
electrophoresis at alkaline & acid pH (Beckman Coulter Appraise system):
More than 18,000 specimens have been analysed to date. Some of the
interesting patterns are Hb DIran, Hb QIndia, Hb E, Hb C, Hb J etc.G-6-PD
screening Osmotic fragility test, Acidified serum (Ham) test, Autohaemolysis
test, Heat instability test for unstable haemoglobin, Heinz bodies,
Haemoglobin F cytochemistry (Kleihauer test), Isopropanol stability test,
Haemosiderin in urine, Serum haptoglobin estimation, Sucrose lysis test,
Methaemoglobin estimation, sickling test for Hb S |
|
Leukemias & lymphomas |
Detailed work-up by CBC, bone marrow
examination, cytochemistry and flow cytometry on acute and chronic leukemias,
myelodysplastic syndromes (MDS), myeloproliferative disorders (MPD) and
lymphoproliferative disorders (LPD) |
|
Flow cytometry |
(Beckman Coulter Epics XL flow
cytometer)
-
Leukemia and
lymphoma immunophenotyping (Acute leukemia panel, CLL panel, Lymphoma
panel)
-
Four-color
single tube CD4 & CD8 counts [HIV panel]
-
Enumeration of
B-, T- & NK cells
-
PNH panel (CD55
& CD59)
-
Flowcytometric
HLA Cross-Match (Donor specific)
-
Stem cell
enumeration based on ISHAGE protocol
-
Platelet
antibody assay
|
|
Molecular Haematology |
Qualitative and quantitative assays
for bcr-abl, TEL-AML1, PML-RARa, JAK2 |
|
Autoimmune disorders |
Collagen disease markers: ENA profile
(screening), ds-DNA, p-ANCA, c-ANCA by ELISA |
|
Other special investigations |
Serum protein electrophoresis (SPE),
Immunofixation electrophoresis (IFE) Urinary protein electrophoresis
(concentration by Minicon filter). – [Beckman Coulter Paragon
electrophoresis and densitometry by Appraise system] |
Clinical
Pathology
Urine: Routine and
microscopic examination
Special - Urobilinogen,
porphobilinogen, haemoglobin, myoglobin, chyle
Body fluids: Apt test,
Ascitic fluid, Cerebrospinal fluid, Gastric aspirate for neutrophils Hemosiderin
in urine, Pericardial fluid, Pleural fluid, Synovial fluid
Useful links
-
www.natboard.edu.in/
- National Board of Examinations
-
www.mciindia.org/ -
Medical Council of India
-
www.ashimagebank.org
- American Society of Hematology – Image Bank
-
www.asheducationbook.org - American Society of Hematology – Education Book
-
www.haematologica.org - Haematologica journal
Accreditation
The laboratory is NABL
accredited.
Quality assurance
Quality assurance is a
major concern of the department. It covers all aspects of laboratory functioning
i.e. pre-analytical, analytical and post-analytical; turn around time;
maintenance of equipment; selection of tests & reagents; standardization &
validation of tests before they are introduced; and training of personnel.
Proposed
Development & Expansion
The department
proposes to enlarge the scope of investigating molecular aspects of haematologic
diseases. The range of flow cytometry tests would also be enhanced. Estimation
of free light chains, a:b
globin chain ratio etc. are some other tests likely to be introduced soon.
Contact us
Laboratory: 42252105 &
42252106
Dr. M. Bhargava: 42252102
Dr Shashi Narayan: 42252103
Dr. Amrita Saraf: 42252101
Dr. Dharma R Choudhary:42251183
Dr. Anil Handoo: 42252104
Residents' room:
42252117
Senior Technical Assistant: 42252107
FAX: +91-11-42252148
Email: hematology@sgrh.com |
