A superspeciality health care hospital
   

            Department of Transfusion Medicine
             (Blood Transfusion Services)

Faculty

Dr. R.K. Saran
Dr. Kamini Khillan

The department assures the supply of safe and quality blood and its products to the patients and provide high quality services.

  • Standards in techniques and quality control are meticulously maintained as laid down in the Drugs and Cosmetics Acts, by the regulatory authority, Director Drugs Controller (India), Ministry of Health & Family Welfare, Government of India, and also internationally recommended methods.

  • The department run round the clock and blood and its products are supplied as and when requested.

  • Blood donation is accepted from voluntary blood donors and the relatives and friends of the patients.

  • Autologous blood donation is also accepted. 

  • The blood donors are educated about the infections transmitted by the transfusion of blood and its products. Persons with high risk behaviour for having infection like (HIV) are advised to exclude themselves from blood donation,

  • 80% of collected blood is processed into components like red cells, platelets and fresh frozen plasma (FFP). The use of components is better and more effective.

Services Provided

  • Blood donors screening and clinical examination

  • Screening of blood for transfusion-transmitted diseases

    • HBV (HBsAg) by ELISA

    • HIV 1 and 2 (anti-HIV 1 & 2) by ELISA

    • HCV (anti-HCV) by ELISA

    • Syphilis

    • Malaria

  • Red Cells serology - tests for ABO & Rh(D)

  • Antibody screening for complete and incomplete antibodies

  • Antihuman globulin test - manual and Gel technology

  • Titre of incomplete antibodies by antihuman globulin test (I.D. Gel technique)
  • Compatibility test is done both for complete & incompelete antibodies
  • Compatibility test for incomplete antibodies is done by Coombs' test (I. D. Gel Technology)
  • Adverse reactions of blood transfusion are investigated

Blood Components Preparation

  • Red cells

  • Platelet concentrate

  • Fresh Frozen Plasma

  • Cryoprecipitate

  • Plasma

Note: Red cells are preserved in preservative solution Adsol and have extended shelf-life upto 42 days. Platelets are reserved in the bags of PVC plasticised with trimalliate (Baxter PL 1240) for 5 days at 20-240C in incubator with agitation.

Leukocytes-Reduced Blood Components

Donors leukocytes present in cellular blood components are linked to wide range of transfusion adverse reactions mainly non-hemolytic reactions, HLA alloimmunisation and platelet refractoriness.

Techniques for preparing leukocytes reduced components are:

  1. New Semi-automated method (using Top and Bottom quadruple bags and OPTI System)

  2. Washing of red cells

  3. Apheresis

Techniques for Preparing Platelet concentrates in this blood bank are:

  1. from platelet rich plasma-platelet yield is 5x1010

  2. from buffy coat - platelet yield is ≥ 6-7X 1010 with leukocytes reduced to ≤ 107 (log 1, 99% leuko-reduction)

  3. plateletapheresis

Platelets are preserved in the bags of PVC plasticised with trimalliate (Baxter PL 1240) for 5 days at 20-240C in incubator with agitation.

APHERESIS

It is done by CS 300 MS Cell Separator (Baxter)

The following procedures are done:

  • Platelet apheresis

  • Granulocytes apheresi

  • Plasma apheresis

  • Therapeutic plasma exchange

  • Peripheral blood stem cell collection and their preservation in DMSO solution at -800C for autologous transplantation.

Merits of Platelet-Apheresis

  • Average yield of platelets is 3x1011 in one unit

  • Plasma volume 200 ml

  • Leukocytes are < 5.5x106 in one units

  • Low risk of alloimmunisation & platelet refactoriness

Common conditions for therapeutic plasma exchange are

  • Myaesthenia Gravis

  • Goodpastures syndrome

  • Acute Gullian-Barre syndrome

  • Waldenstorm's macroglobinaemia

  • Inflammatory / Idiopathic Neuropthy

Plasma Exchange have been done in cases of Myaesthenia Gravis and Inflammatory & Idiopathic neuropathies

Common conditions for peripheral stem harvesting

  • Multiple Myeloma

  • Leuka emias in remission

  • Lymphomas

  • Cancers

Peripheral Stem Cells harvesting for autologous transplantation have been done in cases of multiple myeloma,

Quality Control

  • Computerised data records and achieves

  • Standard Operative Procedures (SOPs) are maintained & followed

  • Quality control protocols are followed according to the Drugs & Cosmetic Acts prescribed by Director Drugs Controller (India), Ministry of Health & Family Welfare (India)

Upgradation of Laboratory

The following equipment are being planned to be added soon:

  • Controlled Temperature Deep Freezer for processing stem cells

  • Equipment for preservation of Stem Cells in Liquid Nitrogen at -1800C

  • Blood Irradiator

  • Another Cell Separator-AMICUS

Academic Activities

  • Attending and participating in the conferences of Indian Society of Blood Transfusion and Immunohaematology

  • Dr. R. K. Saran. attended South Pacific Conference of International Society of Blood Transfusion & Immuohaematology at Bangkok in 2005.
  • Dr. R. K. Saran is the member of the Editorial Board of Transfusion Bulletin of Indian Society of Blood Transfusion & Immunohaematology
  • Teaching the candidates of DNB
  • Teaching and training students of Medical Laboratory Technology of YWCA & Delhi Polytechnic and B. Sc. M. L. T. students

Publications

  1. Transfusion Medicine Technical Manual, 2nd Edition, sponsored by W.H.O., published by D.G.H.S. Ministry of Health and Family Welfare, Govt. of India, 2003
  2. Preparation of Leukocytes - Poor Blood Components - A Newer Technique, Saran, R. K,, Transfusion Bulletin, Indian Society of Blood Transfusion and Haematology, 2004

Facilities to be introduced

  • Peripheral blood stem cells and bone marrow haematopoitic cell harvesting and cryopreserving in liquid nitrogen at -1800C for transplantation
  • Irradiation of blood cellular components to prevent graft verses host disease after transfusion.

Photo gallery


Incubator & Centrifuge for GEL Technique- It is latest technique 
Baxter OPTI System, Baxter An automated blood component extractor

Refrigerated Hereaus Centrifuge 6000i for centrifuging blood at controlled temperature and dividing it into Red cells, plasma and buffy coat
Blood Collection Monitor -Automatically mixing blood with anticoagulant & taking desired amount of blood during collection

Sir Ganga Ram Hospital, Rajinder Nagar, New Delhi 110060, INDIA
Tel: 25735205, 25861463 Fax: 25861002 Email: gangaram@sgrh.com
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